Drosphilia Protocol

Drosphilia Protocol Observations and Procedure: My double mutant locomote argon white eyed and have underlying travel in comparison to the groundless type flies. I plan on determining how these traits atomic number 18 transmitted through genes is as follows: mensuration One: agnate extension I obtained a number of ten double mutant perfect(a) females from my stock ampul by clearing out all the adults and capturing all the new hatched females. I put all three virgin females in a separate vial with six frenzied type males. All, except one, female died within the week with almost of my wild type males flies. I was still equal to take hold the rest alive and larvae had been produced about a week later.

P Generation:                                    Females                           Males Phenotype                           White eye                           Wild instance                                     undeveloped Wings                   Genotype                           Unknown                           Unknown Step both: F1 Generation Ten days after my original insure between the double mutant virgin female flies and the male wild type flies, my F1 generation began to emerge. My totals and observations are as follows: F1 Generation:                                    Females                           Males Phenotype                           Wild Type                           White Eyes                                                                         eagle-eyed Wings Possible Genotype                  Xw+vg X                           Xwvg Y chalk up # Collected Ratios of Phenotypes 1:1 Xw „³ White eye, X-linked recessive allelomorph Xvg „³ fundamental wings, Autosomal Recessive Allele Xwvg „³ White eyes, vestigial wings, X-linked recessive, Autosomal recessive alleles Xw+ „³ Wild type allele Y „³ No alleles for X-linked traits are carried on the Y chromosome Punnett Squares         Xwvg                  Xwvg X++         Xwvg X++         Xwvg X++ Y         Xwvg         Y         Xwvg Y Based on my observations of the F1 Generation, the phenotypes and genotypes of the Parental Generation are likely to be: Parental Generation         :                                                                        Females                           Males Phenotype                           White eyes                           Wild Type                                    Vestigial Wings                   Genotype                           Xwvg Xwvg                           X++ Y The white eyes are found only in the males in the F1 generation, enchantment the vestigial wings did not show up in the males of the F1 generation. All of my females in the F1 generation ended up organism wild type. I believe that the white eyes are a X-linked recessive trait while the vestigial wings are an autosomal recessive trait. My next cross get out be between the F1 females and the F1 males. I can observe the traits in the F2 geneartion to prove or disprove my hypothesis.

Step 3: F2 Generation: As of now, I have done a F1 x F1 cross with no F2 emergence, so the following information I anticipate to occur regarding my F2 generation. I expect all my F1 wild type females are carriers for the X-linked recessive trait. Therefore crossing them with my F1 males should dedicate the following results: F2 Generation                                    Female                                             Male Phenotype                           Wild Type                                    Wild Type                   Genotype                           Xw+vg Xwvg                                    Xw+vg Y Total                                    50% of females                           50% of males Punnett Squares                  Xw+vg Xwvg Xw+vg         Xw+vg Xw+vg Y                  Xw+vg Y          Xw+vg Y                   Plan for abutting Step: Based on the results of my F1 generation and F2 generation, I would be done with my crosses. I would want to obtain a wide-ranging number of F2 generation specimens to increase my accuracy when I do my Chi square test. I will be able to to determine whether or not the traits of my flies are well-nigh linked on the X chromosome. If they are closely linked, I will be able to determine a stand for based on the frequency of recombinants. My final report would let in all the data and calculations in this report.

                          

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