Biochemistry lab I. Introduction In this experiment, lipids from ground nutmeg ar extracted using a combination of firmness of purposes and identify the lipids through chromatography. The use of goods and services of using solvent combinations is to elute the lipids based on their mutual opposition to binding of the silica jelly. The chromatography is performed on a silica gel plate and the use of iodine to visualize the lipids. By scheming the Rf values for each compound and comparing them to the know lipids, we are able to distinguish the lipids within the grounded nutmeg. II.
Procedures Add 5 g crushed nutmeg and 50 mL hexane-isopropanol into a flask and warm for 15 minutes. Remove the extra solvent on a steam bath under a hood while flushing the flask with N2 gas, leaving the crude extract. consult extract. Prepare silica gel column. Add 6 g of silica gel in 20 mL of hexane to read a slurry. Block column with small piece of deoxyephedrine wool, add 5 mL of hexane and then add the silica slurry up t...If you want to get a full essay, show it on our website: Ordercustompaper.com
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